Original Article: Real time reverse transcription (RRT)‐polymerase chain reaction (PCR) methods for detection of pandemic (H1N1) 2009 influenza virus and European swine influenza A virus infections in pigs
نویسندگان
چکیده
reaction (PCR) methods for detection of pandemic (H1N1) 2009 influenza virus and European swine influenza A virus infections in pigs Marek J. Slomka, Anstice L. E. Densham, Vivien J. Coward, Steve Essen, Sharon M. Brookes, Richard M. Irvine, Erica Spackman, Jonathan Ridgeon, Rebecca Gardner, Amanda Hanna, David L. Suarez, Ian H. Brown Avian Virology Workgroup, Virology Department, Veterinary Laboratories Agency (VLA Weybridge), Surrey, UK. Southeast Poultry Research Laboratory, Athens, GA, USA. Correspondence: Dr Marek J Slomka, Avian Virology Workgroup, Virology Department, Veterinary Laboratories Agency (VLA-Weybridge), Woodham Lane, Addlestone, Surrey KT15 3NB, UK. E-mail: [email protected]
منابع مشابه
Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses
Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans. Highly pathogenic avian influenza viruses (HPAI) H5N1 were first identified in Southeast Asia in 1996 and spread to four continents over the following years. The viruses have caused high mortality in chickens and various bird species ...
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In April 2009, the H1N1 pandemic influenza virus emerged as a novel influenza virus. The aim of this study was to compare the performances of several molecular assays, including conventional reverse transcription polymerase chain reaction (RT-PCR), two real-time reverse transcription (rRT)-PCRs, and two multiplex RTPCRs. A total of 381 clinical specimens were collected from patients (223 men an...
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Abstract Background and Objective: The emergence of a novel H1N1influenza A virus of animal origin with transmissibility from human to human poses pandemic concern. Current subtypes of Seasonal influenza A viruses spread in human are influenza A H1N1 influenza A H3N2 and influenza type B viruses. The aim of this study was to determine current strains of the H3N2 and new H1N1 subtypes of influe...
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BACKGROUND Influenza A virus is a leading causative pathogen of human acute respiratory infection. Recently, the co-circulation of pandemic (H1N1) 2009 and seasonal H3N2 viruses was reported, and sporadic cases with reassortant avian H7N9 virus are continually reported in China. We aimed to establish a multiplex one-step real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay ...
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